ABSTRACT
Cerebral injury is a common disease in the pediatric intensine care unit(PICU)and the neonatal intensive care unit (NICU).Video-electroencephalogram examination can help for the etidogical diagnosis,illness monitoring and prognosis assessment.The article is a review about the applications of video-electroencephalogram in common diseases in NICU and PICU.
ABSTRACT
Objective To study the influence of theaflavin on the expressions of TLR4 and release of TNF-αand IL-6 in primari-ly cultured rat airway epithelial cells .Methods Lipopolysacchride(LPS) was adopted to establish the in vitro inflammatory injury model of rat airway epithelial cell ,the TNF-αand IL-6 secreted by the airway epithelial cells and the TLR4 protein level in the air-way epithelial cells were detected with ELISA and the expressions of TLR4 mRNA was detected with RT-PCR .Results LPS sig-nificantly induced the airway epithelial cells to secrete TNF-αand IL-6 ,and enhanced the TLR4 mRNA and TLR4 protein expres-sion(P<0 .01) .Theaflavin could inhibit LPS induced TNF-αand IL-6 secretion and TLR4 gene expression(P<0 .05) ,which was related with the theaflavin concentration (P<0 .05) .Conclusion In rat airway epithelial cell culture ,the anti-inflammatory effect of theaflavin may be decrease the release of inflammatory cytokines via inhibiting TLR 4 gene expression and LPS/TLR4 signal trans-duction pathway .
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OBJECTIVE@#To determine the relation between serum myostatin with body mass index (BMI) and PaO₂/PaCO₂ in men with chronic obstructive pulmonary disease (COPD).@*METHODS@#A cohort of outpatients with stable COPD was evaluated. We evaluated the myostatin, PaO₂/PaCO₂ and BMI, and the patients were stratified by BMI. The plasma level of myostatin and PaO₂/PaCO₂ was measured by high sensitivity ELISA or blood gas analysis.@*RESULTS@#PaCO₂ and myostatin increased significantly compared with those in the control group (P<0.05), but PaO₂ decreased significantly. There was positive correlation between myostatin and PaCO₂ (P<0.05), and negative correlation between myostatin and BMI/FEV1/pred value/PaO₂ (P<0.05).@*CONCLUSION@#Patients with higher myostatin levels had a lower BMI, lower PaO₂ and higher PaCO₂, with poor pathogenetic condition and prognosis. Myostatin may be a potential treatment target in patients with chronic obstructive pulmonary disease.
Subject(s)
Humans , Male , Blood Gas Analysis , Body Mass Index , Monitoring, Physiologic , Myostatin , Blood , Prognosis , Pulmonary Disease, Chronic Obstructive , BloodABSTRACT
OBJECTIVE@#To determine the effect of rebamipide on the expression of Toll-like recepter 4 (TLR4) and TNF-alpha release in pulmonary epithelial cell line A549.@*METHODS@#Lipopolysaccharide (LPS) was used to induce A549 in vitro, which was divided into 4 groups: a control group, a model group(LPS), and 2 intervention groups (10 mg/L rebamipide plus LPS; 30 mg/L rebamipide plus LPS). TNF-alpha release was detected with ELISA and expression of TLR4 was detected with RT-PCR and Western blot.@*RESULTS@#A549 cells were stimulated with LPS and TNF-alpha release was increased compared with the control group (P0.05).@*CONCLUSION@#The antiinflammatory mechanism of rebamipide may be reducing cytokine release by inhibiting TLR4 expression. Rebamipide may be used as a supplementary anti-infection drug.